Figure 3.

VEGFA was a target gene of TUSC8. A, High-throughput sequencing of transcriptome suggested that VEGFA expression was significantly changed after knockdown of TUSC8. B, Dual-luciferase reporter assay confirmed the binding between TUSC8 and VEGFA. C, VEGFA levels in esophageal cancer cell lines detected by qRT-PCR. D, Differential expressions of VEGFA in esophageal cancer tissues and normal ones detected by qRT-PCR. E, TUSC8 level was negatively correlated to VEGFA level in esophageal cancer tissues. F, Kaplan-Meier curves based on VEGFA expressions in esophageal cancer patients. The prognosis was worse in high VEGFA expression group than that in low VEGFA expression group. Data were expressed as mean±SD. *p < 0.05, **p < 0.01, ***p < 0.001.