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. 2021 Aug 28;12(21):6344–6355. doi: 10.7150/jca.61194

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E7FL or E7ΔN interacted with SP1 and formed complex with MALAT1 promoter. A. Co-IP assay for Myc-E7 FL or Myc-E7ΔN and Flag-SP1. E7 or E7ΔN interacted with SP1 in C33A cells. Cells were co-transfected indicated plasmids, following anti-myc IP, coprecipitated SP1 was revealed by anti-flag immunoblotting (93 kDa). Input levels of myc-E7 (22 kDa) or myc-E7ΔN (16 kDa) and flag-SP1 (93 kDa) were also shown. B. UV crosslinking assay for protein purified from E.coli: His-E7 (40 kDa) and His-SP1-C (35 kDa) can form complexes with the MALAT1 promoter DNA in vitro. Arrows indicated the complexes formed (about 75 kDa and 110kDa), and the band of unbound His-E7 was also shown. No complexes band was observed when the mutant probe was used. C. UV crosslinking assay for total C33A cell lysates with indicated plasmid overexpression: Arrows indicated the complexes formed by probe, myc-E7FL/E7ΔN and flag-SP1 (about 104 kDa, 115kDa for the lane of myc-E7FL; 101 kDa, 109 kD for the lane of myc-E7ΔN).