Figure 5.

E7 FL and E7ΔN promote cell proliferation, invasion, and migration, which can be reversed by siMALAT1. A. Wound healing experiments were used to detect the effect of siMALA1 on motility of C33A cells with E7 FL or E7ΔN overexpression. C33A cells were transfected with indicated plasmid, and then wound-healing scratch motility assays were performed. Cell invasion was assessed at 0 and 24 h. Representative images were shown in upper panel. Percentages of wound healing rates were shown in lower panel (***, P < 0.001). B. Colony-forming assay. Upper: C33A cells were transfected the plasmids as indicated. Lower: Statistical analysis of clonogenicity. The number of clonogenicity was presented as the mean ± SD of three different experiments (***, P < 0.001). C. Knockdown of MALAT1 inhibited the migration of C33A cells overexpressing E7FL and E7ΔN. HPV16E7FL or E7ΔN were cotransfected with siMALAT1, after 48h, migrated cells were measured as shown in upper panel. Quantitative assessment of the number of cells migrated to the lower chamber was shown in bottom. D. The proliferation of C33A cells which were transfected indicated plasmids, were tested by CCK8 assay after transfection indicated time.