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. Author manuscript; available in PMC: 2021 Oct 4.
Published in final edited form as: Cell Rep. 2020 Jul 28;32(4):107943. doi: 10.1016/j.celrep.2020.107943

Figure 2. Investigation of pUL37×1 Spatial and Temporal Dynamics across the Replication Cycle of HCMV.

Figure 2.

(A) Schematic representing pUL37×1 localization and HCMV biology, highlighting spatial-temporal changes to organelles. pUL37×1 is translated in the ER around6 hpi, quickly localized to mitochondria and peroxisomes, and remains expressed throughout infection.

(B) Fluorescence microscopy images (z stack maximum projections) of human fibroblast cells infected with pUL37×1-GFP virus (green) and labeled for mitochondria (red, MitoTracker). Arrows indicate likely peroxisomal pUL37×1 localization (pUL37×1 puncta not colocalized with mitochondria). Scale bars are 10 μm.

(C) Western blot of pUL37×1-GFP-infected cell lysates across infection time, with antibodies against viral proteins. Consistent protein loading is indicated by GAPDH.

(D) Workflow schematic of the investigation performed in this study. Two biological replicates were performed for each time point.

See also Table S2