Fig 2.

Increased muscular stamina and decreased atrophy in mice treated with E-selectin/ adeno-associated virus (AAV) gene therapy. A, Representative images of transverse thigh muscle section with hematoxylin-eosin staining. B, Cross-sectional size of individual myocytes, E-selectin/AAV–treated myocytes size set at 1.0. LacZ/AAV–treated myocytes were 30% less than the size of E-selectin/AAV–treated myocytes; the size was significantly larger with E-selectin/AAV treatment (∗∗P < .01). C, Treadmill exhaustion function test in both E-selectin/AAV–treated mice and LacZ/AAV–treated mice on postoperative day (POD) 8. The distance traveled in meters within 60 minutes was measured and compared between groups. The E-selectin/AAV–treated mice had run a significantly greater distance in 60 minutes compared with the LacZ/AAV–treated mice (∗P < .05). Statistical analysis was performed via the two-tailed Student t test. E-selectin/AAV–treated mice, n = 10; LacZ/AAV–treated mice, n = 7.