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Solvent A, 25 mM triethylamine (pH 4.8, adjusted with acetic acid); solvent B, 80%(v/v) acetonitrile; solvent C, methanol.
| Time (min) | Solvent A (%) | Solvent B (%) | Solvent C (%) |
|---|---|---|---|
| 0.0 | 100 | 0 | 0 |
| 1.0 | 78 | 22 | 0 |
| 15.0 | 78 | 22 | 0 |
| 27.0 | 55 | 39 | 6 |
| 27.5 | 53 | 33 | 14 |
| 34.0 | 20 | 10 | 70 |
| 37.0 | 0 | 100 | 0 |
| 57.0 | 0 | 100 | 0 |
