Table 2.
LC-MS/MS conditions for quantification of CBD
| CBD | IS | |
|---|---|---|
| Mass spectrometry parameters for CBD and IS | ||
| Polarity | Negative | Positive |
| Q1 mass, Da | 313.085 | 467.161 |
| Q3 mass, Da | 245.033 | 372.900 |
| Declistering potential, V | −30 | 41 |
| Entrance potential, V | −10 | 10 |
| Collision energy, V | −28 | 13 |
| Collision cell exit potential, V | −17 | 10 |
| Retention time, min | 11.8 | 5.71 |
|
| ||
| >Optimized source parameters | ||
| Curtain gas, psi | 20 | 40 |
| Collision gas, psi | 3 | 5 |
| IonSpray voltage, V | −4,500 | 5,500 |
| Source temperature, ° C | 400 | 400 |
| Ion source gas 1 | 40 | 80 |
| Ion source gas 2 | 70 | 70 |
CBD, cannabidiol; IS, internal standard (clobetasol propionate).