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Trans-synaptically traced interneurons in the upper L4 of a P35 male rat. Concomitant injections of PRV-GFP in the EUS (A) and PRV-RFP in the bladder wall (B) resulted in three main types of labeling: purely green neurons included only in the EUS circuit, purely red cells included only in the bladder circuit and cells expressing green and red fluorescent proteins (yellow color in C=A+B) for cells involved in both circuits.
