FIG. 6.
Presence of the 23-substrate has no impact on nicking of the 12-substrate. (A) The left panel shows the cleavage of the 12-high in the presence of the 23-high over a period of 30 min (lanes 1 through 5). The right panel shows the cleavage of the same 12-high in the absence of any 23-substrates over the same period of time (lanes 6 through 10). (B) The left panel shows the cleavage of the 12-low in the presence of the 23-high over a period of 30 min (lanes 1 through 5). The right panel shows the cleavage of the same 12-low in the absence of any 23-substrates over the same period of time (lanes 6 through 10). Note the accumulation of the nicked products at the bottom of the gel. All other symbols are as in previous figures.