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| Cas variant | Enzyme | Organism | PAM |
|---|---|---|---|
| Cas12 | LbCas12a (or LbaCas12a) | Lachnospiraceae bacterium | 5′-TTTN |
| AaCas12b (or AapCas12b) | Alicyclobacillus acidiphilus | 5′-TTN | |
| Cas13 | LbuCas13a | Leptotrichia buccalis | Not applicable |
| LwaCas13a | Leptotrichia wadei | Not applicable | |
| Cas9 | FnCas9 | Francisella novicida | NGG-3′ |
