TABLE 3.
Readthrough of the PGK-UGA-lacZ construct in the Ssb+ and Ssb− backgrounds
Strain | β-Galactosidase (ng)/mg of total protein
|
Suppression (%) | |
---|---|---|---|
UGA (pUKC819) | Control (pUKC815) | ||
GT174 ([psi−] Ssb+) | 0.5 | 448 | 0.11 |
GT175 ([psi−] Ssb−) | 0.5 | 332 | 0.15 |
GT81-1C ([PSI+] Ssb+) | 7.5 | 713 | 1.05 |
GT146 ([PSI+] Ssb−) | 31.0 | 552 | 5.62 |