Figure 4.

Solution structure and in vivo safety data. (A) Random coil structure with low hairpin content suggests an induced fit mechanism into the SIRPα binding pocket. CD spectra of nS-FF, nS-VV, nS-XX at 5 and 90 °C. Arrows indicate the molar ellipticity at 215–220 and 195 nm, respectively, suggestive of a β-hairpin turn which is lost in nS-XX. (B) Difference plots show that nS-FF and nS-VV agree in terms of structure, consistent with phagocytosis results. (C) Phase-1 type pre-clinical trial of lead peptide, nS-FF: Intravenous injections of nS-FF were done for four consecutive days followed by blood withdrawal 24 h after last injection. Blood parameters show that nS-FF at 1 mg/kg is safe, with no anemia or weight loss.