FIG. 10.

Northern blot analysis of nucleus-encoded p63 (A) and mitochondrially encoded cox2 (B) transcripts. Salt-insoluble (2.5 M NaCl) RNA fractions (25 μg) isolated from embryos before (0 h) and after (1, 5, 15, and 25 h) imbibition were electrophoresed in a 1% agarose gel in 1× MOPS buffer containing 0.66 M formaldehyde; separated RNAs were blotted onto nylon membrane. As probe for p63 transcripts, a portion of the 63-kDa cDNA was amplified with primers 1-14 and 1-15 (Table 1), and the product was labeled with [α-³²P]dATP in the presence of random hexamers. The cox2 probe contained the first exon of the wheat cox2 gene plus flanking 5′ untranslated and 3′ intron sequences. Transcript sizes in the lower panel indicate intron-containing (unspliced) cox2 pre-mRNA (2.9 kb) and mature cox2 mRNA (1.7 kb).