For the UPS1/yeast database, ISB18 database, and the five iterations of the human database, peptides in common between the relevant and irrelevant database were removed from analysis. Any relevant ricin peptide also found in the non-ricin castor plant proteome was considered to be relevant. The number of irrelevant peptides includes the set of neighbor peptides. The peptide counts above are for the target databases, but by construction these counts are nearly identical in the corresponding decoy databases.