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. 2020 Jun 16;1(1):103–118. doi: 10.1002/mco2.11

FIGURE 3.

FIGURE 3

The MET/AXL inhibitor LY2801653 induced cell cycle arrest and inhibited downstream molecules in MKN45 cells. (A‐B) MKN45 cells were treated with either vehicle (DMSO) or increasing concentrations of LY2801653 (10, 20, and 50 nM) for 24 h, stained with PI (DNA content) and analyzed using flow cytometry. Cell cycle arrest at G1 phase was observed in MKN45 cells with lower proportions of cells in S phase as the doses of LY2801653 increased. Representative histograms are shown. All results are representative of three independent experiments. Data are shown as the mean ± SEM. * P  < .05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns: not significant. (C) Western blot analysis for Cyclin A2 and Cyclin D1 expression in MKN45 cells after serial diluted concentrations of LY2801653 treatment. β‐Actin was used as an internal control. (D) MKN45 cells were treated with the indicated doses of LY2801653 (0, 10, 20, 50, 100, 500 nM) for 24 h. Basal levels of MET, AXL, and their downstream molecules were determined by Western blot analysis. Data are representative of three independent experiments