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. 2021 Sep 30;49(9):0300060520980215. doi: 10.1177/0300060520980215

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Gating strategy to identify rare CTCs. The CTCs were identified in peripheral blood samples collected before and after surgery from patients with confirmed CRC stages I to IV. The selection started in both samples with CD45− cells on CD45/SSC plots, followed by CK20+ CK7− cells (CK20/SSC and CK7/SSC plots). Next, debris and dead cells were removed using FSC/SSC and FSC-H/FSC-A plots. Finally, the potential CTC cells were identified.

CTCs, circulating tumor cells; CD45, lymphocyte common antigen; SS, side scatter; CK20, keratin 20; CK7, cytokeratin 7; APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; FS, forward scatter; H, height; A, area.