Figure 4.

Inhibition of WEE1 advances the inhibitory effect of cisplatin on proliferation, invasion, and migration of breast cancer cell lines. A, Silencing efficiency of sh-WEE1-1 and sh-WEE1-2 in MDA-MB-231 cells detected by Western blot. B, mRNA expression of WEE1 detected by RT-qPCR in MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1. C, Protein expression of WEE1 detected by Western blot in MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1. D, Viability of MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1 detected by CCK-8. E, Apoptosis rate of MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1 detected by flow cytometry. F, Migration and invasion of MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1 measured by Transwell assay. G, The protein expression of MMP-2, MMP-9, Bax, and Bcl-2 in MDA-MB-231 cells treated with cisplatin or in combination with sh-WEE1 detected by Western blot. * p < .05, indicates the comparison between two groups. The cell experiment was repeated three times