Figure 6.

Xrn1 binds to osmotic stress-induced genes upon activation in a Hog1-dependent manner. Chromatin immunoprecipitation (ChIP) analysis of Xrn1 binding to the osmotic stress-induced genes STL1 (A) and GRE3 (B) and to the osmotic stress-downregulated gene RPL30 (C) in cells treated with 0.6 M KCl for the indicated time. Wild type and hog1 mutant strains carrying functional Xrn1-Flag (Supplementary Figure S4) were used. Immunoprecipitated samples were analysed for the binding of Xrn1-Flag to the promoter (left panels) and open reading frame (ORF) (right panels) regions of the regulated genes. Data were obtained as described in Figure 5 and normalized respect to the signal at t = 0. Mean and standard deviation from three independent experiments are shown