Figure 8.

Deletion of XRN1 causes high levels of TFIIS and Spt5 recruitment to elongating RNAPII in genes upregulated by osmotic stress. (A) Parallel increase in TFIIS and Rpb3 recruitment at osmotic stress-upregulated STL1 and GRE3 in an xrn1Δ mutant. (B) The amount of Spt5/Rpb3 found at osmotic stress-upregulated STL1 and GRE3 is not changed in an xrn1Δ mutant. (A, B) Binding of HA-TFIIS or Spt5-myc (left panels) and Rpb3 (middle panels) and HA-TFIIS (or Spt5-myc)/Rpb3 ratio (right panels) on STL1 (upper panels) and GRE3 (lower panels) ORFs were analysed by chromatin immunoprecipitation (ChIP) in wild-type (wt) cells and xrn1Δ mutant cells. Cells were treated with 0.6 M KCl for the indicated time. ChIP data were calculated and normalized as described in Figure 5. Mean and standard deviation from three independent experiments are shown