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. 2020 Dec 9;18(10):1434–1444. doi: 10.1080/15476286.2020.1854520

Figure 5.

Figure 5.

Effects of HOTAIR on p21, cyclin D1 and CDK4. (A) Western blot analysis showing the protein levels of p21, cyclin D1 and CDK4 in HL-60 cells treated with PBS and ATRA. β-actin served as a loading control, and (D) relative protein levels (means ± SD, n = 3) of p21, cyclin D1 and CDK4 were measured. (B) Western blot analysis showing the protein levels of p21, cyclin D1 and CDK4 in HL-60 cells transfected with si-HOTAIR and si-NC. β-actin served as a loading control, (E) relative protein levels (means ± SD, n = 3) of p21, cyclin D1 and CDK4 were measured. (C) Western blot analysis showing the protein levels of p21, cyclin D1 and CDK4 in HL-60 cells transfected with HOTAIR plasmid and NC plasmid. β-actin served as a loading control, and (F) relative protein levels (means ± SD, n = 3) of p21, cyclin D1 and CDK4 were measured. * represent P < 0.05