Table 3.
Antibodies used in western blot analysis
Antibodies | Art. No. | Dilution ratio | Reference |
---|---|---|---|
LC3 | ab48394 | 1:2000 | PMID: 30384327 |
Beclin | ab207612 | 1:2000 | PMID: 30384327 |
Atg4B | ab154843 | 1:10000 | PMID: 30384327 |
p62 | ab91526 | 1:100 | PMID: 30384327 |
β-actin | ab179467 | 1:5000 | PMID: 30384327 |
PI3K | ab32089 | 1:1000 | PMID: 30384327 |
p-PI3K | ab182651 | 1:1000 | PMID: 30384327 |
Akt | ab8805 | 1:500 | PMID: 30384327 |
p-Akt | ab8933 | 1:500 | PMID: 30384327 |
PTEN | ab32199 | 1:10000 | PMID: 30313146 |
Note: All antibodies were purchased from Abcam Inc. (Cambridge, MA, USA); LC3, microtubule-associated protein 1 light chain3; PI3K, phosphatidylinositol 3-kinase; p-PI3K, phosphorylated PI3K; Akt, protein kinase B; p-Akt, phosphorylated Akt; PTEN, phosphatase and tensin homologue deleted on chromosome 10.