Table 3.

KEGG pathway analysis for EV proteome

Term in KEGG pathway	Corrected p-value
Upregulated in ΔprsA1/A2 mutant
Aminoacyl-tRNA biosynthesis AlaS, ArgS, AsnC, AspS, GatA, GatB, GltX, GlyS, HisS, LysS, MetS, Fmt, PheT, SerS, TrsA	3.8E-11
Mismatch repair DnaE, DnaX, HolA, LigA, MutL, MutS, MutS2, PolC, PcrA, RecJ Spy1212	2.9E-8
Pyrimidine metabolism CarA, CarB, DnaE, DnaX, HolA, NrdD, NrdE1, NrdE2, PolC, PyrB, PyrC, PyrH, Spy1212, ThyA, Udk	3.0E-7
Purine metabolism DnaE, DnaX, GuaB, GuaC, HolA, NrdD, NrdE1, NrdE2, PurA, PolC, Pyk, RelA, Spy0298, Spy1212, Xpt	1.5E-6
DNA replication DnaE, DnaG, DnaX, HolA, HolB, LigA, PolC, Spy1212	3.8E-5
Pyruvate metabolism AccC, AckA, AcoA, AcoB, OadA2, PflD, Ppc, Pyk, Spy0041	3.3E-4
Ribosome RpsC, RplA, RplB, RplC, RplD, RplF, RplJ, RplO, RplU, RplV, RplW	6.2E-4
Alanine, aspartate and glutamate metabolism AsnA, CarA, CarB, GlmS, PurA, PyrB	6.9E-4
Selenoamino acid metabolism MetB, MetS, Meth1, Metk2, Spy0261, Spy1106	1.5E-3
Butanoate metabolism AcoA, AcoB, PflD, MvaS1, Spy0041, Spy0553	5.0E-3
Peptidoglycan biosynthesis MurA, MurC, MurD, MurE, MurF, MurZ	1.3E-2
Dnregulated in ΔprsA1/A2 mutant
Fructose and mannose metabolism FruA, ManM, ManN, Pfk, PtsB, PtsC, PtsD, Spy0438, Spy1342	2.2E-6
Phosphotransferase system (PTS) FruA, AgaD,, ManM, ManN, LacE, PtsB, PtsC, PtsD, ScrA, Spy1512	3.1E-5
Arginine and proline metabolism ArcA, ArcB, ArcC, GlnA, Spy0941, Spy1053, Spy1378	1.7E-4
ABC transporters MalC, MalD, OppB, OppC, PotB, SagG, SagI, SrtF, Spy0624, Spy0980	2.4E-4
Pentose phosphate pathway Pfk, Pgi, Rpe, Tkt, Spy0996, Spy1342, Spy1487	2.7E-4
Amino sugar and nucleotide sugar metabolism GlcK, ManM, ManN, Pgi, PtsB, PtsC, PtsD, Spy1342	2.9E-4
Glycolysis/Gluconeogenesis Eno, GapN, GlcK, Pfk, Pgi, Plr, Spy1342	2.3E-3
Galactose metabolism AgaD, GlcK, LacE, Pfk, Spy1342, Spy1512	1.1E-2

*Pathways with corrected p-value less than 0.05 and involved more than five protein numbers are considered significant.