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. 2021 Sep 27;2021:6773260. doi: 10.1155/2021/6773260

Figure 3.

Figure 3

Oxidative stress effects of BJIGT combined with riluzole in TDP-43-expressed stress granular cells. TDP-43 stress granular cells were pretreated with BJIGT (100 μg/mL), riluzole (50 μM), and combined BJIGT (100 μg/mL)/riluzole (50 μM) for 6 h before treatment of SA (150 μM) for 18 h. (a) Representative data of HO1 and Bax expression in TDP-43 stress granular cells. SA-induced oxidative stress-related proteins (HO1 and Bax) were reduced by BJIGT/riluzole in TDP-43-expressed stress granular cells. Tubulin was used as loading control. (b) Quantification of immunoblots. Bars represent the means ± SEMs of more than three independent experiments. ∗P < 0.05 vs. the SA-treated control group. (c) TDP-43 stress granular cells were treated with SA (150 μM) for 18 h in the absence or presence of BJIGT (100 μg/mL), riluzole (50 μM), and combined BJIGT (100 μg/mL)/riluzole (50 μM), and then incubated with 5 μM DCFH-DA. Cells were captured with a fluorescent microscope (40× magnification). (d) Relative fluorescence was quantified with a microplate reader. Bars represent the means ± SEMs of more than three independent experiments. ∗P < 0.05 and  ∗∗p < 0.01 vs. the SA-treated group. C, control; SA, sodium arsenite; BJIGT, Bojungikgi-tang; RI, riluzole.