Figure 7.

Probiotic V and Met alone or in combination prevents ethanol-mediated oxidative stress in Caco-2 monolayer cells. mRNA expression of (a) CYP2E1 and (b) NOX. The gene expression levels were measured after normalizing against 18S. Values are expressed as mean ± SD of three individual experiments. Statistical analysis: one-way ANOVA followed by Tukey's post hoc test. ^###p < 0.001 and ^####p < 0.0001 compared to the control group. ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001 compared with the ethanol-fed group; ^ap < 0.05 and ^aap < 0.01 compared with the E + probiotic V group; ^bp < 0.05 and ^bbp < 0.01 compared with the E + Met group.