Table 2.
The list of Rattus norvegicus primers used for the quantification of mRNA expression levels in qRT-PCR.
| Sr. no. | Name of the gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|
| 1 | 18S | GTTGGTTTTCGGAACTGAGGC | GTCGGCATCGTTTATGGTCG |
| 2 | ZO-1 | TCGGAGCTCGGGCATTATTC | CAGGGCACCATACCAACCAT |
| 3 | Occludin | CAACGGCAAAGTGAATGGCA | CTTTCCCCTTCGTGGGAGTC |
| 4 | Nrf-2 | CAGAGTTTCTTCGCCAGAGG | TGAGTGTGAGGACCCATCG |
| 5 | HO-1 | CAAATCCCACCTTGAACACA | CGACTGACTAATGGCAGCAG |
| 6 | CYP2E1 | TCAATCTCT GGACCCCAACTG | GCGCTCTGCACTGTGCTTT |
| 7 | NOX | TGACAGTGATGTATGCAGCAT | CAGCTTGTTGTGTGCACGCTG |
| 8 | CHOP | ACCACCACACCTGAAAGCA | AGCTGGACACTGTCTCAAAGG |
| 9 | Grp78 | CCGTAACAATCAAGGTCTACGA | AAGGTGACTTCAATCTGGGGTA |
| 10 | TNF-α | TCTCATTCCTGCTCGTGGCG | GGTGAGGAGCACGTAGTCGG |
| 11 | IL-6 | TTGACAGCCACTGCCTTCCC | CGGAACTCCAGAAGACCAGAGC |
| 12 | IL-10 | TGCCTTCAGTCAAGTGAAGAC | AAACTCATTCATGGCCTTGTA |
| 13 | AMPK | GCTGTGGATCGCCAAATTAT | GCATCAGCAGAGTGGCAATA |
| 14 | SREBP-1c | TCTGCCTTGATGAAGTGTGG | AGCAGCCCCTAGAACAAACA |
| 15 | FASN | CCTCAGTCCTGTTATCACCCGA | GCTGAATACGACCACGCACTA |
| 16 | ACC | CCTTCTTCTACTGGCGACTGAG | TAAGCCTTCACTGTGCCTTCC |
| 17 | GPR109A | ACTTTCTGGTGATAAACGGCAAGA | GACTGTCAGGCCGATGGTG |
| 18 | SLC5A8 | AGCCAGCACTCAGCGTATTT | TTTGAGCTCCAATTCCAACC |