Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 30;18(10):1233–1238. doi: 10.1038/s41592-021-01277-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, Schematic of MALDI MS workflow for high-throughput single-DCV measurements. b, Brightfield image of DCVs distributed on a glass slide. c, Identification of primary objects (DCVs) using image-processing software. d, Masked image output of identified DCVs. The colored spots represent primary objects (DCVs) recognized as ‘foreground’ and are marked with a maximal pixel intensity value. Anything not identified as an object is treated as ‘background’ and is set to a zero-pixel intensity value. e, Brightfield image of mechanically induced DCV release from the AG. f, DCVs plated on a glass slide for relative DCV density estimation using brightfield microscopy. Each slide held DCVs from three animals (biological replicates) and a total of three slides (technical replicates) were prepared, where 598 DCVs were measured. g, Mass spectra demonstrating the coverage of AG peptides detected in single-DCV measurements. h, AGPA1 (XP_012945143.1) and AGPB1 (XP_012945142.1) prohormone sequences with corresponding MALDI MS-detected peptides italicized and font colored to match the annotated spectra in g. AG peptide assignments were validated using LC–MS/MS and performed on AG extracts (n = 3). The peptides detected by LC–MS/MS are underlined in black. a.u., arbitrary units.