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. 2021 Sep 28;204:112141. doi: 10.1016/j.envres.2021.112141

Fig. 1.

Fig. 1

Phylogenetic analysis of the spike genes used for genetic engineering. The genes used for genetic engineering are those described in the NIH Grant 1RO1 Al 110,964–01 (https://www.documentcloud.org/documents/21055989-understanding-risk-bat-coronavirus-emergence-grant-notice), i.e. WV1, WV16, SHC014 and Rs4231. The spike protein gene of MERS-CoV was used as outgroup to root the tree. Sequences were aligned using MUSCLE in the SeaView package (Gouy et al., 2010). The phylogenetic tree was built using the maximum likelihood method with GTR-G-I evolutionary model and 500 bootstrap repeats.