FIG. 3.

Protein oxidative damage under normal growth conditions of wild type and grx single and double mutants. MATa strains were employed. Cultures of wild type (CML235) and single and double mutants were grown in YPD liquid medium at 30°C until an optical density at 600 nm of 1 was reached. The crude extracts obtained were analyzed by Western blotting with anti-DNP antibodies (B). A parallel run stained with Coomassie brilliant blue is shown in panel A. Each lane contained 20 μg of total protein. Asterisks mark the identified transketolase band (see text for details).