FIG. 7.

Northern blot analyses of GRX3, GRX4, and GRX5 expression. Samples were taken at different stages of the population growth curve in YPD liquid medium at 30°C (A) or after treatment of mid-exponential-phase cells (at 30°C except for heat shock) with KCl (0.5 M), hydrogen peroxide (0.4 mM), menadione (2 mM), or heat shock for the indicated times (B). Small nuclear U2 mRNA is shown as the loading control. Numbers under the lanes indicate the mRNA levels for each time point, relative to the mid-exponential-phase sample. For heat shock analysis, the time zero sample corresponds to exponential cultures at 25°C.