TABLE 1.
Strains used in this worka
| Strain | Relevant genotype | Comments |
|---|---|---|
| CML235 | Wild type | |
| CML236 | Wild type | |
| MML15 | MATa grx3::kanMX4 | Deletion in CML235 |
| MML16 | MATα grx3::kanMX4 | Deletion in CML236 |
| MML17 | MATa grx4::kanMX4 | Deletion in CML235 |
| MML18 | MATα grx4::kanMX4 | Deletion in CML236 |
| MML19 | MATa grx5::kanMX4 | Deletion in CML235 |
| MML20 | MATα grx5::kanMX4 | Deletion in CML236 |
| MML37 | MATa grx3::kanMX4 grx5::kanMX4 | From a cross, MML15 × MML20 |
| MML39 | MATa grx4::kanMX4 grx5::kanMX4 | From a cross, MML17 × MML20 |
| MML41 | MATa grx3::kanMX4 grx4::kanMX4 | From a cross, MML15 × MML18 |
| MML42 | MATα grx3::kanMX4 grx4::kanMX4 | From a cross, MML15 × MML18 |
| MML44 | MATa grx2::LEU2 | Deletion in CML235 |
| MML45 | MATa grx2::LEU2 grx3::kanMX4 | From a cross, MML44 × MML16 |
| MML47 | MATa grx2::LEU2 grx4::kanMX4 | From a cross, MML44 × MML18 |
| MML57 | MATα grx5::kanMX4 tetO2(GRX5)::kanMX4 | From MML20, by transformation with the pCM224 cassette (4) |
| MML58 | MATa grx3::kanMX4 grx4::kanMX4 grx5::kanMX4 tetO2(GRX5)::kanMX4 | From a cross, MML41 × MML57 |
| MML59 | MATa grx2::LEU2 grx3::kanMX4 grx4::kanMX4 | From a cross, MML42 × MML44 |
a
Wild-type strains CML235 (MATa ura3-52 leu2Δ1 his3Δ200) and CML236 (MATα ura3-52 leu2Δ1 his3Δ200) are spores from FY1679 (diploid, MATa/α ura3-52/ura3-52 leu2Δ1/+ his3Δ200/+ trp1Δ63/+; from B. Dujon, Pasteur Institute, Paris, France). The other strains have been obtained during this work.