TABLE 2.
Enzyme activities and protein oxidation levels in grx mutantsa
| Strain | Relevant genotype | Doubling time (min)b | Glutaredoxin activityc | Glutathione reductase activityc | Carbonyl contentd
|
||
|---|---|---|---|---|---|---|---|
| Control | Menadione | Hydrogen peroxide | |||||
| CML235 | Wild type | 90 ± 4 | 51 ± 4 | 102 ± 10 | 0.69 ± 0.05 | 1.28 ± 0.05 | 0.96 ± 0.05 |
| MML15 | grx3 | 94 ± 1 | 30 ± 3 | 101 ± 6 | 0.78 ± 0.06 | 1.53 ± 0.05 | 1.10 ± 0.05 |
| MML17 | grx4 | 92 ± 5 | 28 ± 3 | 97 ± 6 | 0.75 ± 0.03 | 1.38 ± 0.05 | 1.08 ± 0.05 |
| MML19 | grx5 | 145 ± 11 | 22 ± 1 | 96 ± 6 | 0.99 ± 0.07 | 2.10 ± 0.05 | 1.63 ± 0.05 |
| MML37 | grx3 grx5 | 208 ± 9 | 19 ± 4 | 101 ± 10 | 1.16 ± 0.10 | ND | ND |
| MML39 | grx4 grx5 | 147 ± 3 | 21 ± 4 | 99 ± 9 | 1.15 ± 0.12 | ND | ND |
| MML41 | grx3 grx4 | 150 ± 16 | 44 ± 7 | 98 ± 12 | 0.89 ± 0.09 | ND | ND |
| MML44 | grx2 | 88 ± 2 | 14 ± 3 | ND | 0.81 ± 0.03 | 1.50 ± 0.10 | 0.95 ± 0.05 |
| MML45 | grx2 grx3 | 92 ± 1 | 19 ± 4 | ND | 0.89 ± 0.11 | ND | ND |
| MML47 | grx2 grx4 | 90 ± 2 | 16 ± 4 | ND | 1.09 ± 0.14 | ND | ND |
| MML59 | grx2 grx3 grx4 | 159 ± 7 | 8 ± 2 | ND | 1.25 ± 0.15 | ND | ND |
a
Values are shown as means ± standard deviations. Three independent determinations were done in all cases. ND, not determined.
b
In exponential cultures in YPD liquid medium at 30°C.
c
Nanomoles of NADPH oxidized per minute per milligram of protein.
d
Nanomoles of carbonyl groups per milligram of protein, in untreated cells (control) or cells treated for 1 h with 20 mM menadione or 2.5 mM hydrogen peroxide.