Fig. 3. Verification of anti-SARS-CoV-2 activity of asunaprevir.

(A) Vero E6 cells were either mock-infected or infected with SARS-CoV-2 (MOI = 0.1) in the absence or presence of asunaprevir for 1 h. At 24 h postinfection, SARS-CoV-2 nucleoprotein levels were determined by an immunoblot assay. (B) Vero E6 cells were infected with SARS-CoV-2 in the presence of various concentrations of asunaprevir. At 24 h postinfection, total RNA was extracted and the IC₅₀ value was determined on the basis of SARS-CoV-2 RNA levels measured by qRT-PCR. (C) Vero E6 cells were infected with SARS-CoV-2 in the presence of various concentrations of remdesivir. At 24 h postinfection, the IC₅₀ value was determined as described in panel B.