Table 3. Gender specific changes in PFAS levels.
A. Liver and circulating plasma samples from n=7 mice from each treatment group was analyzed by a non-targeted metabolomics approach by Metabolon Inc. Three of the five PFAS included in the PFAS mixture were analyzed using LC-MS. Relative fold change between various treatment groups is given for hepatic and plasma levels of PFOS, PFOA, and PFHxS. Statistical significance for the interaction and main effects of PFAS exposure and gender was determined by two-way ANOVA analysis. B. Absolute plasma concentrations (ug/mL) of 5 PFAS included in the exposure mixture. Plasma was extracted from male and female mice (n=3 per group) exposed to PFOS, PFOA, PFNA, PFHxS, and GenX and levels were quantitated by electrospray ionization mass spectrometry. Red indicates a significant relative fold change increase (p<0.05); Blue indicates a significant relative fold change reduction (p<0.05).
| A. | |||||
|---|---|---|---|---|---|
| Relative PFAS Levels | |||||
| Biochemical Name | Fold Change Vehicle Female vs. Vehicle Male | Fold Change PFAS Female vs. PFAS Male | Fold Change PFAS vs. Vehicle | Overall PFAS Treatment q-value | Sex-PFAS Treatment Interaction q-value |
| Relative Hepatic PFAS Levels | |||||
| Perfluorooctanesulfonate (PFOS) | 0.30 | 0.92 | 188.03 | q<0.01 | q<0.01 |
| Perfluorooctanoate (PFOA) | 0.30 | 2.43 | 1513.94 | q<0.01 | q<0.01 |
| Perfluorohexanesulfonic acid (PFHxS) | 0.25 | 1.30 | 705.46 | q<0.01 | q=0.12 |
| Relative Plasma PFAS Levels | |||||
| Perfluorooctanesulfonate (PFOS) | 1.00 | 0.77 | 627.07 | q<0.01 | q=0.28 |
| Perfluorooctanoate (PFOA) | 1.25 | 1.96 | 10270.75 | q<0.01 | q=0.40 |
| Perfluorohexanesulfonic acid (PFHxS) | 0.80 | 0.96 | 6629.5 | q<0.01 | q=0.88 |
| B. | |||||
| Absolute Plasma PFAS Levels | |||||
| Biochemical Name | PFAS Female Average Concentration (ug/mL) | PFAS male Average Concentration (ug/mL) | Overall Average Concentration (ug/mL) | Fold Change PFAS Female vs. PFAS Male | p-value |
| PFOA | 23.4 +/− 9.0 | 7.3 +/− 1.8 | 15.4 +/− 10.5 | 3.2 | p=0.04 |
| L-PFOS | 13.8 +/− 2.3 | 22.5 +/− 4.1 | 18.1 +/− 5.6 | 0.6 | p=0.03 |
| L-PFHxS | 22.0 +/−8.3 | 25.6 +/− 5.8 | 23.8 +/−6.7 | 0.9 | p=0.57 |
| PFNA | 33.7 +/− 7.6 | 40.9 +/− 10.6 | 37.3 +/− 9.1 | 0.8 | p=0.40 |
| HFPO-DA (GenX) | 0.19 +/− 0.11 | 0.10 +/− 0.04 | 0.14 +/− 0.09 | 1.8 | p=0.27 |