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Aggregation of strains supplemented with exogenous factors.
| Aggregate formation with supplements | |||
|---|---|---|---|
| Strain | None | CM | cAMP |
| gα2 − | − | − | − |
| gα2−(Gα2D−) | − | − | − |
| gα2−(Gα2) | + | + | + |
Cell droplets on non-nutrient agar plates were treated with developmental buffer (none), conditioned medium (CM), or 100 μM cAMP every hr for 6hr. Representative images of cell droplets after 11 hr are displayed in Fig. S2.
