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. 2021 Oct 5;7:90. doi: 10.1038/s41421-021-00301-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a qPCR analysis of Il6 mRNA from control or histone deacetylase (HDAC) 1–3 inhibitor CI-994-pretreated peritoneal macrophages infected with H37Rv, H37RvΔFadA, or H37Rv(ΔFadA + FadA) strains for 4 h (MOI = 1) (mean ± SEM). b qPCR analysis of Il6 mRNA from control or OSS_128167, a selective inhibitor that increases the acetylation of H3K9-pretreated peritoneal macrophages infected with H37Rv, H37RvΔFadA, or H37Rv(ΔFadA + FadA) strains for 4 h (MOI = 1) (mean ± SEM). c ChIP-seq analysis of histone H3 acetylation at the lysine 9 residue (H3K9Ac) for the Il6 promoter of peritoneal macrophage infected with H37Rv or H37RvΔFadA strains for 4 h (MOI = 1) with SimpleChIP Enzymatic Chromatin IP Kit. d ChIP-qPCR (mean ± SEM) analysis of H3K9Ac for the Il6 promoter of peritoneal macrophage infected with negative control, H37Rv, H37RvΔFadA, or H37Rv(ΔFadA + FadA) strains for 4 h (MOI = 1). Data in a, b, d represent one experiment with at least three independent replicates. One-way (d) or two-way (a, b) ANOVA with Bonferroni’s multiple comparisons test were used for statistical analysis.