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. 2021 Oct 4;12:5808. doi: 10.1038/s41467-021-26075-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic workflow of PAL experiments. Probes were incubated with cell lysates and irradiated with UV light to initiate photo-crosslinking. Labeled proteins were affinity-purified using desthiobiotin (DTB) as affinity tag. Eluted fractions were digested and analyzed by LC-MS/MS and label-free quantification (LFQ). b Functionalized, non-hydrolysable Ap₃A and Ap₄A derivatives used as PAL probes (PALP). c Unlabeled, non-hydrolysable Ap₃A and Ap₄A derivatives and a control substance (con-1) lacking the Ap_nA scaffold served as controls in PAL experiments.