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. 2021 Oct 4;11:19674. doi: 10.1038/s41598-021-99134-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Conditional ablation of Relb in T cells does not impair their homeostasis at steady-state. Thymus, spleen and peripheral lymph nodes (LN) from CD4^cre (control) and CD4^creRelb^F/F (Relb-cKO) mice were analyzed by flow cytometry. (a) Quantification of live TCR-β⁺CD4⁺ and CD8⁺ cells. (b) Proportion of Ki67⁺ in CD4⁺Foxp3^- (Tconv) cells. (c) Representative dot plots in the spleen and cumulative data of CD44 and CD62L expression in Tconv cells. T_CM: T central memory; T_E_M: T effector memory. (d) Cytokine expression by Tconv cells following restimulation with PMA/ionomycin. Each dot represents an individual mouse from 3-5 experiments. Mean + /- SEM is shown. ns: non-significant.