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. 2021 Oct 4;11:19674. doi: 10.1038/s41598-021-99134-x

Figure 2.

Figure 2

Normal proliferative capacity and cytokine expression of Relb-deficient T cells in vitro. CTV-labeled naïve Tconv cells were stimulated with soluble anti-CD3 + mitomycin C-treated feeders. (a,b) After 4 days of culture, cells were restimulated with PMA/ionomycin and analyzed by flow cytometry. (a) Representative CTV profile and cumulative proliferation index of live cells. (b) Cytokine expression following restimulation with PMA/ionomycin. (c) ELISA analysis of supernatants after 24 h of culture. Each dot represents an individual mouse from 5 experiments. Mean + /- SEM is shown. ns: non-significant.