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. 2021 Oct 4;12:5810. doi: 10.1038/s41467-021-25689-y

Fig. 5. Homo- and co-ROP of GluNCA and CysNCA.

Fig. 5

a, b Synthetic scheme (a) and SEC (b) characterization of PLG via the direct ROP of GluNCA at a feeding monomer/initiator ratio of 20/1(black trace) and 50/1 (red trace). The Mn values reported here were obtained from a SEC equipped with a multi-angle light scattering detector and a refractive index detector. mobile phase: 50 mM NaH2PO4, pH = 9 in H2O; flow rate of 0.5 mL/min; dn/dc of PLG = 0.1184 ± 0.002859 mL/g). c, d Synthetic scheme (c) and SEC (d) characterization of the block polymer PLG-block-P(EG3-Glu). eg Synthetic scheme (e), CD (f), and SEC in DMF (g) characterization of P(EG3-Glu)-branch-PCys (red trace) via the ROP of EG3GluNCA and CysNCA. The blue trace in (g) represents the partially degraded polypeptides after native chemical ligation (NCL).