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. 2021 Sep 13;11(19):9452–9469. doi: 10.7150/thno.62376

Figure 3.

Figure 3

TNF-α induces neuronal necroptosis is RIPK1-dependent. (A) Immunoblotting of cell lysates from the SH-SY5Y cells (left panels) or primary neurons (right panels) probed with the indicated antibodies. (B) Immunoblotting of soluble and insoluble proteins (see methods) from SH-SY5Y cells (left panels) or primary neurons (right panels) probed with the indicated antibodies. β-Actin as a loading control. (C) Immunoblotting of cell lysates from the SH-SY5Y cells (left panels) or primary neurons (right panels) with or without TSZ and Necrostain-1 treatments. (D) Quantitation of cell viability from SH-SY5Y cells and primary neurons with or without indicated treatments. (E) Quantitation of PI-positive cells from cell cultures with indicated treatments as in (D). (F) Immunoblotting of the SH-SY5Y cells transduced with lentiviral particles encoding eGFP (as control) or RIPK1 shRNA. (G) Quantitation of PI-positive cells from SH-SY5Y cells expressed eGFP or RIPK1 shRNA with or without indicated treatments. ap < 0.05, difference with control group; bp < 0.05, difference with TNF-α treated group; cp < 0.05, difference with TSZ treated group; dp < 0.05, difference with eGFP group. Data represent mean ± SEM, n=3~5 independent experiments; analysis was performed by one-way ANOVA test.