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. 2021 Sep 13;11(19):9397–9414. doi: 10.7150/thno.57037

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Snhg1 improved adult cardiac function post-MI. (A) Schematic of the MI experiments in adult mouse hearts injected with AAV9. Echo, echocardiography. IF, immunofluorescence. (B) Detection of Snhg1 expression in sham operated and infarcted adult mouse hearts by ISH analysis (n = 4). (C-D) Immunofluorescence for Ki67 and pH3 in adult mouse hearts 14 days after MI (n = 6). Arrows indicate positive CMs. (E-F) Immunofluorescence for TUNEL and IB4 in adult mouse hearts 14 days after MI (n = 6). Arrows indicate positive cells or vessels. (G) Immunohistochemistry for CD31 in adult mouse hearts 14 days after MI (n = 6). (H) Masson's trichrome-stained sections of adult mouse hearts 28 days after MI and quantification of infarct size (n = 6). (I) Echocardiography analysis of adult mouse hearts at 1, 14, and 28 days after MI (n = 10).