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. 2021 Sep 13;11(19):9397–9414. doi: 10.7150/thno.57037

Figure 8.

Figure 8

Mutant Snhg1 inhibited Snhg1-induced CM proliferation. (A) Construction of the overexpression vectors Adv-Snhg1-WT and Adv-Snhg1-MU. (B-C) RT-qRCR analysis of Snhg1 levels and Western blotting analysis of PTEN, PI3K, p-AKT, AKT, and c-Myc protein levels in isolated CMs (n = 4). (D) EdU staining of P7 CMs (427 CMs from 5 mice in the Adv-Snhg1-WT group, 387 CMs from 5 mice in the Adv-Snhg1-MU group). Arrows indicate positive CMs. (E) Aurora B immunofluorescence staining of P7 CMs (326 CMs from 5 mice in the Adv-NC group, 406 CMs from 5 mice in the Adv-Snhg1-WT group, 354 CMs from 5 mice in the Adv-Snhg1-MU group). Arrows indicate positive CMs. (F-H) Representative images from time-lapse videos of P7 CMs transduced with Adv-Snhg1-WT (F), Adv-Snhg1-MU (G), or Adv-NC (H). Representative images from time-lapse videos of P7 CMs transfected with Adv-Snhg1-WT (F), Adv-Snhg1-MU (G), or Adv-NC (H). CMs grown for 36 h immunostained for cTnT and stained with DAPI.