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. 2021 Sep 21;11(19):9605–9622. doi: 10.7150/thno.63763

Figure 6.

Figure 6

c-Myc preferentially drives the expression of OXPHOS genes in quiescent cells. (A, B) EGFP-p27highmCherry-Ki67low quiescent (Q) and cycling (C) cells isolated from dually edited Mel-RM (Mel-RM.DE) and A375 (A375.DE) cells undergoing serum starvation were subjected to qPCR-ChIP assays. Values are mean ± SDs; n = 3 (*P < 0.05; **P < 0.01, two-tailed Student's t-test). (C, D) EGFP-p27highmCherry-Ki67low quiescent (Q) and cycling (C) cells isolated from Mel-RM.DE and A375.DE cells transfected with the indicated luciferase plasmids undergoing serum starvation were subjected to luciferase reporter assays. Values are mean ± SDs; n = 3 (*P < 0.05; **P < 0.01, two-tailed Student's t-test). (E) Whole cell lysates from EGFP-p27highmCherry-Ki67low quiescent (Q) cells isolated from Mel-RM.DE and A375.DE cells carrying an inducible c-Myc shRNA system with or without treatment with Dox undergoing serum starvation were subjected to Western blotting. n = 3. (F) EGFP-p27highmCherry-Ki67low quiescent (Q) cells isolated from Mel-RM.DE and A375.DE cells carrying an inducible c-Myc shRNA system transfected with the indicated luciferase plasmids with or without treatment with Dox were subjected to serum starvation followed by luciferase reporter assays. Values are mean ± SDs; n = 3 (***P < 0.001; ****P < 0.00001, two-tailed Student's t-test). (G) Whole cell lysates from EGFP-p27highmCherry-Ki67low quiescent (Q) cells isolated from Mel-RM.DE and A375.DE cells transfected with the indicated plasmids undergoing serum starvation were analyzed using Western blotting. n = 3. (H) EGFP-p27highmCherry-Ki67low quiescent (Q) cells isolated from Mel-RM.DE and A375.DE cells transfected with the indicated plasmids undergoing serum starvation were subjected to luciferase reporter assays. Values are mean ± SDs; n = 3 (*P < 0.05; **P < 0.01, ***P < 0.001; ****P < 0.00001, two-tailed Student's t-test). (I, J) MM200 cells transfected with the indicated luciferase plasmids undergoing serum starvation were subjected to luciferase reporter assays. Values are mean ± SDs; n = 3 (*P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student's t-test).