Figure 5.

Onvansertib inhibits the formation and growth of CAL33 RR metastases in zebrafish. (A-D) Zebrafish embryos (n = 28) were injected with CAL33 RR (labelled in red) into the perivitelline space and immediately treated with onvansertib (50 nM) for 72 h. Representative images of zebrafishes are shown at 24 h, 48 h and 72 h (A). Quantification and representative images of no, few and high local metastases in the head after 72 h of treatment (B). Percentage of zebrafishes with tumor cells in the tail (distant metastasis) at 24 h were quantified (C). Area of distant metastases at 72 h were quantified (D). (E-F) Zebrafish embryos (n = 48) were injected with CAL33 RR (labelled in red) into the perivitelline space. 24 h later, zebrafish embryos with metastases were treated with onvansertib (50 nM) for 48 h. Representative image of zebrafish are shown (E) and variations of the metastases area before and after treatment were quantified (F). Statistics were performed using an unpaired Student's t test: ** p < 0.01, *** p < 0.001.