Figure 7.

Onvansertib on human tumor sections from HNSCC patients. (A) Tumors from HNSCC patients surrounded by agarose and tumor sections were done using a vibratome. These sections were cultured in a specific medium and treated for 4 days with onvansertib or/and cisplatin or/and radiotherapy. Analyses were then performed (n = 6). (B) Necrosis of biopsies' sections assessed using HES (Hematoxylin Eosin Saffran) staining. (C-I) Biopsies' sections treated with onvansertib (50 nM and 100 nM), cisplatin (3 μM and 10 μM) or radiotherapy (2 Gy) (C, E and H), or treated with onvansertib alone or in combination with cisplatin and radiotherapy (D, F and H). (C-D) ATP quantification of biopsies' sections. (E-G) Quantification of cell death by apoptosis by TUNEL assays on biopsies' sections. (H-I) Pictures and quantification of cell proliferation evaluated by KI-67 staining. Statistics were performed using ANOVA test: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and unpaired Student's t test: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.