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. 2021 Sep 27;11(19):9705–9720. doi: 10.7150/thno.61731

Figure 4.

Figure 4

Upregulation of stemness-associated factors in metastatic cells. (A) Western blot analysis of SOX2 (n = 3) and real-time PCR analysis (n = 6) of three stemness factors (Student's t-test, * P < 0.05, ** P < 0.01, *** P < 0.001). (B) Western blot analysis of BCAT1 and SOX2 in human primary lung cancer cells (H441) and lung cancer cells isolated from lymph node metastasis (H661), n = 3. (C) mRNA levels of SOX2-regulated genes from a previous RNA-seq data, n = 1. (D) Immunofluorescent imaging of SOX2 (green) and DAPI staining in L0, L2 and L6 A549 cells (magnification: 1000 ×, scale bar: 10 μm). (E) Bar graph of SOX2-positive cells from immunocytochemistry studies, n = 3. (F) FACS analysis of CD133 expression in control (L0) and L2 and L6 cells expressing shBCAT1. Cells were stained with CD133-PE, co-stained with 7-ADD. (G) Bar graph of FACS group data (Student's t-test of data from F). * P < 0.05, ** P < 0.01, **** P < 0.0001, n = 3~5.