Skip to main content
. 2021 Sep 27;11(19):9705–9720. doi: 10.7150/thno.61731

Figure 6.

Figure 6

BCAT1 regulates the expression of miR200c and its target SOX2. (A) Target sequence of several microRNAs including miR200c at the 3'UTR of SOX2 gene (Targetscan.org). (B) Correlation analysis of BCAT1 mRNA and miR200c expression from TCGA LUAD data, correlation efficient (Cor) and P value are shown. (C) Expression of miR200c in A549 cells (* P < 0.05, n = 6). (D) Real-time PCR analysis of miR200c in L2 and L6 cells expressing shBCAT1 and scrambled shRNA, n = 6. The efficiency of BCAT1 knockdown is shown. (E) Expression of miR429 and miR21-5p in A549 cells with or without BCAT1 knocking down. (F) Effect of DM-α-KG treatment on SOX2 expression in L2 and L6 cells. The cells were treated with 4 mM DM-α-KG for 4 h. (G) Effect of DM-α-KG treatment (4 mM 4 h) on miR200c expression in L2 and L6 cells.