Fig. 2.

E2 upregulates SCF in primary Sertoli cells. Primary human Sertoli cells were treated with vehicle control (a) or 100-ng/ml E2 (b). Immunofluorescence staining of SCF (red) was performed after 48 h. The nuclei are stained with DAPI (blue). Scale bar: 50 μm. c Primary human Sertoli cells were treated with 0-, 50-, 100-, 150-, 200-, or 500-ng/ml E2 for 1 h, and the relative expression of SCF mRNA was measured by qRT-PCR. β-actin was used as a housekeeping gene. *P < 0.05 vs. vehicle control. d Primary human Sertoli cells were treated with 100-ng/ml E2 for 0, 15, or 30 min, or 1, 3, or 24 h, and the relative expression of SCF mRNA was measured by qRT-PCR. β-actin was used as a housekeeping gene. *P < 0.01 vs. control group (0 h). ^&&P < 0.01 vs. 100-ng/ml E2 treatment for 30 min