Fig. 7.
E2 suppresses apoptosis of human fetal SSCs. Human fetal SSCs co-cultured with Sertoli cells were treated with vehicle control, 100-ng/ml E2, or 100-ng/ml E2 + 10−7 M ICI 182780 for 1, 3, or 5 days, stained for TUNEL, and examined by fluorescence microscopy. Representative image of TUNEL staining (green) (white arrow) and DAPI (blue) in control (a), E2-treated (b), and E2 + ICI treated (c). Scale bar: 50 μm. d The percentage of apoptotic nuclei in human fetal SSCs relative to the total cell count (200 random cells) under different treatments. Values are presented as the mean ± SEM of three separate experiments. *P < 0.01 vs. control