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. 2021 Jul 2;37(10):1381–1396. doi: 10.1007/s12264-021-00737-1

Fig. 4.

Fig. 4

Effects of blocking ASIC2 or TRPV1 on stretch-activated currents in cells overexpressing ASIC2 and TRPV1. A Upper traces, effects of negative pressure on currents from HEK293T cells co-expressing ASIC2 and TRPV1 (DMSO control group). Lower traces, expanded 1-s segments from 0 (a) and −30 mmHg (b). B Upper traces, channel activation in HEK293T cells co-expressing ASIC2 and TRPV1 after treatment with 100 μmol/L benzamil. Lower traces, expanded 1-s segments from 0 (a) and −30 mmHg (b). C Upper panels, effects of negative pressure on currents from HEK293T cells co-expressing ASIC2 and TRPV1 treated with 20 μmol/L capsazepine. Lower traces, expanded 1-s segments from 0 (a) and −30 (b) mmHg. D Quantification of the pressure effect on NPo of channels expressed in HEK293T cells before and after treatment with 100 μmol/L benzamil or 20 μmol/L capsazepine. E All-point histogram analysis of channel opening events for a cell co-expressing ASIC2-TRPV1 from (a) the DMSO control group, and (b) after treatment with 20 μmol/L capsazepine (**P <0.01, NS not significant vs 0 mmHg, paired t-test). NPO, total single-channel open probability; A + T, co-expression of ASIC2 and TRPV1.